Analysis Note

Low-end CV% Range: 5 - 9%|Low-end CV% Average: 7%

Application

Research Sub CategoryCardiovascular Disease

Used to detect/quantify:G-CSF

The sensitivity of Single Molecule Counting (SMC^®) technology gives the SMC^® G-CSF Immunoassay Kit unparalleled performance in the quantification of G-CSF in human EDTA plasma.

Research CategoryCardiovascular Disease

Components

G-CSF Coated BeadsStandard DiluentG-CSF Detection AntibodyAssay BufferHuman G-CSF StandardSMC^® Human G-CSF Immunoassay Kit Instructions10X Wash BufferElution Buffer BBuffer D

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

General description

The SMC^® G-CSF Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Granulocyte-Colony Stimulating Factor (G-CSF) is an endogenous hematopoietic agent activated and expressed by many types of cells, including macrophages, tumor cells, fibroblasts, and endothelial cells. One of the main functions of G-CSF is to regulate formation of neutrophils during hematopoiesis, which are important for proper immune function. For example, in cancer patients reduced hematopoeisis from chemotherapy results in low neutrophil production, which jeopardizes the immune system, leaving the body susceptible to infectious attacks. G-CSF therapy has been shown to restore hematopoiesis in cancer and leukemic patients by stimulating white blood cell production. Interestingly, G-CSF has also demonstrated effectiveness in improving cardiac function following a myocardial infarction.

Legal Information

SMC is a registered trademark of Singulex